Dissection of pelvic autonomic ganglia and associated nerves in male and female rats

MM Bertrand; JR Keast

Journal article

Dissection of pelvic autonomic ganglia and associated nerves in male and female rats

MM Bertrand, JR Keast

Journal of Visualized Experiments | MyJove Corporation | Published : 2020

DOI: 10.3791/60904

Abstract

The bilateral major pelvic ganglia (MPG; synonym, pelvic ganglia) are the primary source of postganglionic sympathetic and parasympathetic neurons innervating pelvic organs of rodents; the functionally equivalent structure in humans is the inferior hypogastric plexus. The major pelvic ganglia also provide the route by which lumbar and sacral sensory axons reach the pelvic organs. These complex, mixed ganglia can prove challenging to identify and dissect for further experimental study of normal autonomic mechanisms or to establish preclinical models of disease, injury or visceral pain. Here we describe a protocol to access and visualize these ganglia and their associated nerve tracts. We prov..

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University of Melbourne Researchers

Janet Keast Author

Related Projects (1)

Understanding functional connectivity of sensory and motor pathways to specific regions of the lower urinary tract.

This foundational project will produce mesoscopic and microscopic functional anatomical maps of the sensory and autonomic (motor) neurons th..

Grants

Awarded by National Institutes of Health

Funding Acknowledgements

Research reported in this publication was supported by the Office of the Director, National Institutes of Health, Stimulating Peripheral Activity to Relieve Conditions (SPARC) Program, Award Number OT2OD023872. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Dr. Bertrand's fellowship in Dr. Keast's laboratory was financed by: The University Hospital of Nimes, the faculty of medicine of Montpellier-Nimes, The Association Francaise de Chirurgie (AFC), The Societe Interdisciplinaire Francophone d'UroDynamique et de Pelviperineologie (SIFUD-PP) and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement No PCOFUND-GA-2013-609102, through the PRESTIGE programme coordinated by Campus France.